Omega-3 fatty acids in gene expression, tissue remodeling, premature delivery, brain function and eicosanoids.

Honors B.S. 1965 Joint Honors in both Chemistry and Physics,
University of London, England, U.K.

Ph.D. 1973 Biochemistry, Montana State University,
Bozeman, MT 59715

July 1987 - present Professor, Department of Food Science and Human Nutrition, Colorado State University, Fort Collins, CO 80523.

March 1991 - present Graduate Faculty, Department of Pediatrics, University of Colorado Health Sciences Center, 4200 E 9th. Avenue, Denver, CO 80262

December 1993- present Professor, Department of Physiology, Colorado State University, Fort Collins, CO 80523

Outstanding paper presentation Award at the 88th AOCS (American Oil Chemists' Society) Meeting, May 11-14, 1997, Seattle WA, for the paper presentation "Cellular Oxidation and Gene Expression".

American Society for Nutritional Sciences (ASNS)/Proctor & Gamble Graduate Student Award Oral Competition. Iwakiri, Y., Sampson, D.A. and Allen, K.G. D. A possible mechanism of anticarcinogenic effects of conjugated linoleic acid. Yasko Iwakiri (joint PhD advisee of Kenneth G.D. Allen and David A. Sampson) was selected as one of 12 graduate students in the US to have all of her expenses paid to attend Experimental Biology 2000 meeting (SanDiego CA, April 15-18, 2000) and to present her doctoral research as a finalist for the ASNS/ Proctor & GambleAward. See page 54-55 of the Program Session 148.

• FN 150 Survey of Human Nutrition
• FN 470 Integrative Nutrition and Metabolism
• FN 550 Advanced Nutritional Sciences I (protein, vitamin, mineral metabolism)
• FN 551 Advanced Nutritional Sciences II (carbohydrate, lipid, energy metabolism)
• FN 650A Recent Developments in Human Nutrition - protein, vitamins, minerals
• FN 650B Recent Developments in Human Nutrition - carbohydrates, lipids, energy

Hankenson, K.D., Watkins, B.A., Schoenlein, I.A., Allen, K.G.D. and Turek, J.J. Omega-3 fatty acids enhance ligament fibroblast collagen formation in association with changes in interleukin-6 production. Proc. Soc. Exptl. Biol. Med. 223: 88-95, 2000.

Park, Y., Allen, K.G.D., and Schultz, T.D. Modulation of MCF-7 breast cancer cell signal transduction by linoleic acid and conjugated linoleic acid in culture. Anticancer Res. 20: 669-676, 2000.

Watkins, B.A., Li, Y., Allen, K.G.D., Hoffmann, W.E and Seifert, M.F. Dietary ratio of n-6/n-3 PUFA alters the fatty acid composition of bone compartments and biomarkers of bone formation in rats. J. Nutr. 130: 2274-2284, 2000.

Watkins, B.A., Li,Y., Rogers, L.L., Hoffmann, W.E., Iwakiri, Y., Allen, K.G.D. and Seifert, M.F. Effect of red palm oil on bone tissue fatty acid composition and histomorphometric parameters. Nutr. Res. 21: 199-213, 2001.

Allen, K.G.D. and Harris, M.A. The role of n-3 fatty acids in gestation and parturition. Proc. Soc. Exptl. Biol. Med. 226: 498-506, 2001.

Harris, M.A., Hansen, R.A., Vidsudhiphan, P., Koslo, J.L., Thomas, J.B., Watkins, B.A. and Allen, K.G.D. Effects of conjugated linoleic acids and docosahexaenoic acid on rat liver and reproductive tissue fatty acids, prostaglandins and matrix metalloproteinase production. Prostaglandins Leukotrienes and Essential Fatty Acids 65:23-29, 2001.

McGregor, J.A., Allen, K.G.D., Harris, M.A., Reece, M.S., Wheeler, M., French, J.I. and Morrison, J. The omega-3 story: Nutritional prevention of preterm birth and other adverse pregnancy outcomes. Obstetrical and Gynecological Survey 56: S1-S13, 2001.

Iwakiri, Y., Sampson, D.S. and Allen, K.G.D. Supression of cyclooxygenase-2 and inducible nitric oxide synthase expression by conjugated linoleic acid in murine macrophages. Prostaglandins Leukotrienes and Essential Fatty Acids 67: 435-443, 2002.

Turek, J.J., Watkins, B.A., Schoenlein, I.A., Allen, K.G.D., Hayek, M.G. and Aldrich, C.G. Oxidized lipids depress canine growth, immune function, and bone formation. J. Nutr. Biochem. 14: 24-31, 2003.

Yu, L., Perret, J., Parker,T. and Allen, K.G.D. Enzymatic modification to improve the water-absorbing and gelling properties of psyllium. Food Chem. 82: 243-248, 2003.

Watkins, B.A., Li, Y., Romsos, D.R., Hoffman, W.E., Allen, K.G.D. and Seifert, M.F. CLA and Bone Modeling in Rats. In: Advances in Conjugated Linoleic Acid Research, Volume 2, pp 218-250 (Sebedio, J-L., Christie, W.W. and Adlof, R. Eds.) American Oil Chemists Society (AOCS) Press, Champaign IL, 2003.

Allen, K.G.D., Bristow, S.J. and Yu, L. Hypolipidemic effects of modified psyllium preparations. J. Agric. Food Chem. 52: 4998-5003, 2004.

Troxell, H., Anderson, J., Auld, G., Marx, N., Harris, M.A., Reece M.S. and Allen, K.G.D. Omega-3 for Baby and Me: Material Development for a WIC intervention to Increase DHA Intake During Pregnancy. Maternal and Child Health Journal 9: 189-197, 2005.

Perez, M.A., Hansen, R.A., Harris, M.A. and Allen, K.G.D. Dietary docosahexaenoic acid alters pregnant rat reproductive tissue prostaglandin and matrix metalloproteinase production. J. Nutr. Biochem. 17: 446-453, 2006.

Kelavkar, U.P., Hutzley, J., Dhir, R, Kim, P.Y, Allen, K.G.D. and McHugh, K. Prostate tumor growth and recurrence can be modulated by the omega (?)-6 : ?-3 ratio in diet: Athymic mouse exnograft model simulating radical prostatectomy. Neoplasia, 8: 112-124, 2006.

Hansen, R.A., Harris, M.A., Pluhar, G.E., Motta, T., Brevard, S., Ogilvie, G.K., Fettman, M.J. and Allen, K.G.D. Fish oil decreases matrix metalloproteinases in knee synovia of dogs with inflammatory joint disease. J. Nutr. Biochem. 19: 101-108, 2008.

Anderson, J., Auld, G., Allen, K.G.D., Harris, M.A. Educational strategies designed to improve intake of docosahexaenoic acid-rich foods and increase gestational length in a WIC population. J. Am. Diet. Assoc. In press, 2009.

Harris, M.A., Reece, M.S., McGregor, J.A., Manchego, J.M. and Allen, K.G.D. Possible roles of maternal and perinatal long-chain fatty acids in preterm birth. In: Lipids in Infant Nutrition (Huang, Y-S and Sinclair, A.J. Eds) AOCS (American Oil Chemists Society) Press, Champaign IL. Chapter 1, pp 1-18, 1998.

Lippman, H.E., LeBouteiller, L., Allen, K.G.D. and Watkins, B.A. Fatty acid modulation of bone-formation markers in MC3T3-E1 osteoblast-like cells. Presented at Experimental Biology 2000 Meeting, San Diego, CA, April 15-17, 2000. FASEB J. 14, A225 (Abstract 163.13), 2000.

Iwakiri, Y., Sampson, D.A. and Allen, K.G.D. A possible mechanism of anti-carcinogenic effects of conjugated linoleic acid. Presented at Experimental Biology 2000 Meeting, San Diego, CA, April 15-17, 2000. FASEB J. 14, A253 (Abstract 176.15), 2000.

Watkins, B.A., Li, Y., Allen, K.G.D., Rogers, L.L., Lepine, A.J., Reinhart, G.A and Seifert, M.F. Dietary ratio of n-6/n-3 alters bone measurements in rats. Presented at Experimental Biology 2000 Meeting, San Diego, CA, April 15-17, 2000. FASEB J. 14, A251 (Abstract 176.6), 2000.

Iwakiri, Y., Wilson, J.J., Sampson, D.A. and Allen, K.G.D. Depression of Cu,Zn-superoxide dismutase (Cu,Zn-SOD) activity by copper marginal and deficient diets alters prostaglandin E2 (PGE2) and nitric oxide (NO) as well as cyclooxygenase-2 (COX-2) expression in rat peritoneal macrophages. Presented at Experimental Biology 2000 Meeting, San Diego, CA, April 15-17, 2000. FASEB J. 14, A773 (Abstract 533.4), 2000.

Harris, M.A., Allen, K.G.D., Iwakiri, Y., Hansen, R.A., Visudhiphan, P., Koslo, J.L., Thomas, J.B. and Keller, A. Conjugated linoleic acid docosahexaenoic acid inhibit rat reproductive tissue prostaglandin, matrix metalloproteinase production, and cyclooxygenase expression. Presented at 91st AOCS (American Oil Chemists Society) Annual Meeting, San Diego, CA, April 25-28, 2000. Session Nutrition and General Health II, page S94 of Abstract book (Inform 11, S94, 2000) and page 83 of Program.

Flass, T.E., Wilson, J.W., Harris, M.A. and Allen, K.G.D. In utero and early developmental omega-3 fatty acids, brain fatty acids and learning in rats. . Presented at Experimental Biology 2001 Meeting, Orlando, FL, March 31 - April 4, 2001. FASEB J. 15, A1227 (Abstract # B324A), 2001.

Allen, K.G.D., Perez, M.A., Hansen, R.A. and Harris, M.A. N-3 fatty acids alter eicosanoid and matrix metalloproteinase mediators of parturition in rats. Presented at Experimental Biology 2003 Meeting, San Diego, CA, April 11-15, 2003. FASEB J. 17, A1118 (Abstract # 695.1), 2003.

Hansen, R.A., Allen, K.G.D., Pluhar, E.G., Ogilvie, G.K., Fettman, M.J. and Harris, M.A. N-3 fatty acids decrease inflammatory mediators in arthritic dogs. Presented at Experimental Biology 2003 Meeting, San Diego, CA, April 11-15, 2003. FASEB J. 17, A330 (Abstract # 200.3), 2003.

Allen, K.G.D. A role for dietary n-3 fatty acids in gestational length. Presented at The Institute of Food Technologists (IFT) Annual Meeting and Food Expo, July 12-16, 2003, Chicago IL, as part of Session 82: Symposium on Dietary n-6/n-3 Fatty Acid Balance. Abstract 82-3, page 207 of 2003 IFT Annual Meeting Technical Program Abstracts.

Harkins, J.M., Hazan, A.M., Allen, K., Penner, K.M., Pestka, J.J. and Claycombe, K.J. Modulation of IL-6 expression and secretion in adipose tissue in vitro and in vivo by n-3 fatty acids. Presented at Experimental Biology 2004 Meeting, Washington DC, April 17-24, 2004. FASEB J. 18, A866 (Abstract # 587.4), 2004.

1. Dietary copper, hepatic glutathione redox and cholesterol synthesis.
USDA National Research Initiative (NRI) Competitive Grant Program
95-00981 Principal Investigator
9/1/95 -8/31/99 $216,000

2. Dietary copper: Hepatic gene expression and liver redox
USPHS NIH NIDDK Co-Investigator
9/1/96 - 8/31/99 $430,538

3. The effect of n-3 long chain polyunsaturated fatty acids on inflammatory mediators and matrix metalloproteinase levels in dogs with osteoarthritis after surgical correction of acute anterior cruciate ligament rupture
Ralston Purina Co., St. Louis MO Co-Investigator
5-36001
6/01/00 -5/31/02 $45,986

4. Premature birth and docosahexaenoic acid enriched functional foods
USDA, Initiative for Future Agriculture and Food Systems Program (IFAFS)
USDA Grant # 00-52102-9633 Principal Investigator
9/15/00 - 9/30/04 $1,200,000
10. Current Research Projects:

1. Premature birth and docosahexaenoic acid enriched functional foods
USDA, Initiative for Future Agriculture and Food Systems Program (IFAFS).
This project is examining the effect of supplemental DHA on pregnancy duration (gestational length and premature delivery) in pregnant women. We are also determining the influence of DHA on prostaglandins E2 and F2a , hormones involved with parturition and utrine, cervical and placental remodeling at delivery.

2. N-3 polyunsaturated fatty acids and Human Health and Disease, Colorado Agricultural Experiment Station and North Central Region Multistate Project NC 1167. This project is determining the importance of both the forms (DHA, EPA versus linolenic acid) and amounts of n-3 fatty acids (0.7% of calories versus 2.0% of calories) on gestational length, the expression of tissue remodeling enzymes, macrophage metabolism in heart disease and cancer, and brain function-learning. All of these studies are with experimental animals and cell culture systems.